METHOD FOR MEASURING BONDING ACTIVITY OF ANTIBODY WHICH MIMICS ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXIC ACTIVITY

摘要

Disclosed is a method for evaluating a binding activity of an antibody to both of an antigen and an Fc receptor in a simple manner. Specifically disclosed is a method for measuring a binding activity of an antibody to both of an antigen or an antigen epitope and an Fc receptor or a fragment thereof, which comprises the steps of: mixing the antibody or the antibody epitope each labeled with either one of a donor and an acceptor which can cause a fluorescence resonance energy transfer, the Fc receptor or the fragment thereof each labeled with the other one of the donor and the acceptor and the antibody; irradiating the mixture with a light having a wavelength capable of exciting the donor; and measuring the amount of fluorescence in the mixture. Further disclosed are: a method for predicting the ADCC activity of an antibody; a method for controlling the quality of an antibody; a method for producing an antibody; a method for screening an antibody; and a kit for use in each of the above-mentioned methods.