METHOD FOR RAPIDLY DESIGNING PHARMACEUTICAL PREPARATIONS FOR PREVENTING VIRAL INFECTION

摘要

Certain embodiments of the present invention include a method for rapidly designing pharmaceutical preparations for preventing viral infection that have adapted to growth in various culturing systems but maintain antigenic similarity to the original virus. The method may include serial passaging a plurality of instances of a targeted virus strain from an infected first animal in respective droplets in second animal cells, in parallel synthesizing antibodies that neutralize the targeted virus strain in a third animal, and selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the targeted virus strain.

主权项

1. A method for rapidly designing pharmaceutical preparations for preventing viral infection, the method comprising:
(a) serial passaging a plurality of instances of a targeted virus strain from an infected first animal in respective droplets in second animal cells, wherein the targeted virus strain adapts to the second animal cells to form second animal cell-adapted viral lineages; (b) in parallel synthesizing antibodies that neutralize the targeted virus strain in a third animal; and (c) selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the targeted virus strain.