METHODS AND COMPOSITIONS FOR PRODUCING STEM CELL DERIVED DOPAMINERGIC CELLS FOR USE IN TREATMENT OF NEURODEGENERATIVE DISEASES

摘要

The present disclosure relates to methods for producing dopaminergic cells and evaluating their functionality. When pluripotent human embryonic stem cells are cultured on plates coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 in cell culture medium containing a GSK3 inhibitor and a TGF-β inhibitor as well as timely administered fibroblast growth factor, desired neural cells are produced at far higher rates. Useful cell culture kits for producing such dopaminergic cells are also described herein, as are methods of using such cells for stem cell therapy.

主权项

1. A method for providing caudalized dopaminergic progenitor cells with a high probability of successful graft outcome, comprising:
plating embryonic stem cells on a first substrate coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 to produce dopaminergic progenitor cells; identifying dopaminergic progenitor cells that express caudal ventral midbrain markers; and isolating the identified dopaminergic progenitor cells from the other dopaminergic progenitor cells on the substrate to obtain the caudalized dopaminergic progenitor cells with a high probability of successful graft outcome.