发明名称 |
AGONISTIC ANTIBODY DIRECTED AGAINST HUMAN THROMBOPOIETIN RECEPTOR |
摘要 |
<p>This invention provides an agonist antibody to a human thrombopoietin receptor (alias: human c-Mpl). More particularly, this invention provides an agonist antibody to a human thrombopoietin receptor, wherein the agonist antibody comprises: antibody constant regions comprising (1) amino acid sequences in a heavy chain constant region and a light chain constant region of a human antibody, (2) an amino acid sequence of a heavy chain constant region with a domain substituted between human antibody subclasses, and an amino acid sequence of a light chain constant region of a human antibody, or (3) amino acid sequences comprising a deletion(s), substitution(s), addition(s), or insertion(s) of one or several amino acid residues in the amino acid sequences of (1) or (2) above ; and antibody variable regions capable of binding to and activating a human thrombopoietin receptor; and wherein the agonist antibody has the properties: (a) that the antibody induces colony formation at a concentration of 10,000 ng/ml or lower as determined by the CFU-MK colony formation assay using human umbilical-cord-blood-derived CD34+ cells; and (b) that the antibody has a maximal activity at least 50% higher than that of PEG-rHuMGDF and an 50% effective concentration (EC50) of 100 nM or less in the cell proliferation assay using UT7/TPO cell. Also provided is a pharmaceutical composition for treating thrombocytopenia comprising said antibody.</p> |
申请公布号 |
EP2006380(A1) |
申请公布日期 |
2008.12.24 |
申请号 |
EP20070739974 |
申请日期 |
2007.03.20 |
申请人 |
KIRIN PHARMA KABUSHIKI KAISHA |
发明人 |
KAI, MASAYUKI;MOTOKI, KAZUHIRO;KATAOKA, SHIRO;YOSHIDA, HIDEAKI;HAGIWARA, TETSUYA |
分类号 |
C12N15/09;A61K39/395;A61P7/00;A61P7/04;A61P43/00;C07K16/22;C07K16/24;C07K16/28;C07K16/46;C12N15/02;C12P21/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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