| 摘要 |
PROBLEM TO BE SOLVED: To provide a bag for cell cultivation capable of preparing cell containing liquid in which floating cell, multipotent stem cell etc. are contained at a high concentration and living cells are included at a high ratio.SOLUTION: A bag for cell cultivation comprises a bag shape container made of tetrafluoroethylene (TFE)-hexafluoropropylene (HFP) copolymer, in which calculated average roughness (Ra) in surface roughness of inner surface of the bag shape container is 3.5 to 6.5nm, root-mean-square roughness (RMS) in surface roughness of the inner surface of the bag shape container is 4.5 to 8.0nm, and surface free energy in inner surface of the bag shape container is 16.5 to 18.5 (mJ/m). When human marrow mesenchymal stem cells (hMSC cells) are cultured in the bag shape container, multipotent stem cells are efficiently induced due to improvement in floating condition, thus preparation of suspension of multipotent stem cell may be carried out at high concentration and at high ratio in living cell, enabling providing cell suspension for transplantation in regenerative therapy of high quality.SELECTED DRAWING: None |
