METHODS AND COMPOSITIONS OF MOLECULAR PROFILING FOR DISEASE DIAGNOSTICS

摘要

The present invention relates to compositions, kits, and methods for molecular profiling and cancer diagnostics, including but not limited to gene expression product markers, alternative exon usage markers, and DNA polymorphisms associated with cancer. In particular, the present invention provides molecular profiles associated with thyroid cancer, methods of determining molecular profiles, and methods of analyzing results to provide a diagnosis.

主权项

1. A method of detecting a thyroid condition in a subject comprising:
(a) obtaining a sample of thyroid from said subject; (b) assaying expression levels of three or more gene expression products in a sample of thyroid tissue obtained from said subject, which gene expression products correspond to at least three genes or corresponding Transcript Cluster ID Nos selected from EIF1AY, NTRK2, IGF2BP2, SLC5A8 and TTF1; (c) using a computer to classify said sample of thyroid tissue as benign or normal by applying an algorithm to said expression levels from (a), wherein said classifying is based on an expression level in said sample of thyroid tissue of said at least three genes or corresponding Transcript Cluster ID Nos selected from EIF1AY, NTRK2, IGF2BP2, SLC5A8 and TTF1; (d) generating an electronic report upon classifying said sample of thyroid tissue, which electronic report is indicative of said sample of thyroid tissue being benign or normal; and (e) outputting said electronic report that is indicative of said sample of thyroid tissue being benign or normal.