CALIBRATION APPARATUS AND METHOD FOR FLUORESCENT IMAGING

摘要

A fluorescence standard for identifying variations in illumination during imaging has a composite fluorescent laminar structure, which fluoresces in response to light in an approximate wavelength. The fluorescent object has at least two areas with different fluorescent response, e.g., a first made from a strongly luminescing material, such as GG420 filter glass having an attenuating effect on the fluorescent response. Variations in illumination during imaging with a camera are detected by placing the standard before the camera during imaging. Each captured image may contain the fluorescent response of the standard in different images and be compared to identify any response changes due to variations in illumination. The variations in illumination can then be remediated by adjusting the source of illumination, the camera or ambient lighting. The images can be normalized through digital image processing.