| 摘要 |
The present invention relates to a dumbbell-structure oligonucleotide (DSO), a nucleic acid amplification primer comprising the same, and a nucleic acid amplification method using the same and, more specifically, to a method for multiple gene amplification and single-nucleotide polymorphism analysis using a dumbbell-structure oligonucleotide capable of excluding non-specific amplification products prior to binding to a template in a first cycle in performing a polymerase chain reaction. The present invention suppresses undesired amplification products at room temperature using a dumbbell structure oligonucleotide (DSO) generated by adding any nucleotide sequence designed to allow the 5'-terminal oligonucleotide and the 3'-terminal oligonucleotide to complimentarily bind to each other, a 3'-terminal template dependent specific nucleotide sequence, and a universal nucleotide pair for linking the two nucleotide sequences, prior to binding to a template in every first cycle at the time of the polymerase chain reaction (PCR), and as a result, efficiently increases sensitivity and specificity through the reduction in non-specific amplification products, thereby achieving the innovation of the gene amplification method. |
