| 摘要 |
A method and Kit for performing concurrent identification testing and antimicrobial susceptibility testing from broth culture are described. Broth incubation is generally 4 to 6 hrs providing adequate numbers of microorganisms for inoculating a multi-chambered kit plate comprising enriched, differential, selective, differential-selective, single-purpose and susceptibility media. Several dilutions are prepared from the cultured broth, for inoculation of the kit plate. The more dilute concentration produces individual colonies of microorganisms, for identification testing. The isolation makes an initial isolation step unnecessary. The heavier concentration dilution provides a lawn inoculation for antimicrobial susceptibility testing (directly proportional to a Kirby-Bauer AST) as well as other identification tests. Antimicrobial susceptibilities are valid even when several different microorganisms coexist in the same test chamber. The method is fast for bacteria, providing identification and susceptibility data in 24hrs. The kit is complete, except for an incubator and microscope. The method is simple to perform and can be utilized almost anywhere. Novel agar-based media formulated with Iota carrageenan provides for a significantly long shelf life of the test media. |
