摘要 |
The purpose is to provide a method for suppressing decomposition of a probe in a nucleic acid detection reaction solution in a probe-assisted nucleic acid detection method, and to enable stable genetic analysis. A nucleic acid detection method wherein a probe is used and a chelating agent is also present in the nucleic acid detection reaction solution; the chelating agent being, in particular, phenanthroline, nitrilotriacetic acid (NTA), maleic acid, citric acid, tricine, ethylenediaminetetraacetic acid (EDTA), trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA), glycol ether diaminetetraacetic acid (EGTA), tartaric acid, nicotinamide, hydroxyiminodiacetic acid (HIDA), or phytic acid. |