| 发明名称 |
METHODS FOR TESTING T CELL PRIMING EFFICACY IN A SUBJECT |
| 摘要 |
The present invention relates to methods for testing T cell priming efficacy in a subject. In particular the present invention relates to an in vitro method for testing T cell priming efficacy in a subject comprising the steps of a) providing sample from the subject, b) culturing the sample in a medium which induces the differentiation of dendritic cells, c) maturing the dendritic cells obtained at step a) in presence of an amount of at least one antigen and an amount of at least one cytokine or ligand suitable for the activation of a pathogen recognition receptor, d) priming and expanding the T cells present in the sample and e) analyzing the func tionality of the primed T cells. |
| 申请公布号 |
US2016341718(A1) |
申请公布日期 |
2016.11.24 |
| 申请号 |
US201515112475 |
申请日期 |
2015.01.20 |
| 申请人 |
INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) ;UNIVERSITÉ PARIS - SUD |
发明人 |
MALLONE Roberto;APPAY Victor;LISSINA Anna |
| 分类号 |
G01N33/50;A61K39/00;C12N5/0783;C12N5/095;C12N5/0784 |
主分类号 |
G01N33/50 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. An in vitro method for testing T cell priming efficacy in a subject comprising the steps of a) providing a sample from the subject, b) culturing the sample in a medium which induces differentiation of dendritic cells, c) maturing the dendritic cells obtained at step a) in presence of an amount of at least one antigen and an amount of at least one cytokine or at least one ligand suitable for the activation of a pathogen recognition receptor, d) priming and expanding T cells present in the sample and e) analyzing the polyfunctionality of primed T cells obtained at step d). |
| 地址 |
Paris FR |
