ENHANCED PRODUCTION OF LIPIDS CONTAINING POLYENOIC FATTY ACIDS BY VERY HIGH DENSITY CULTURES OF EUKARYOTIC MICROBES IN FERMENTORS

摘要

PROBLEM TO BE SOLVED: To provide a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids, and to provide a process for producing eukaryotic microbial lipids.

SOLUTION: The present invention provides a process for growing eukaryotic microorganisms which are capable of producing at least about 20% of their biomass as lipids and a method for producing the lipids. Preferably the lipids contain one or more polyenoic fatty acids. The process comprises adding to a fermentation medium comprising eukaryotic microorganisms a carbon source, preferably a non-alcoholic carbon source, and a limited nutrient source. Preferably, the carbon source and the limited nutrient source are added at a rate sufficient to increase the biomass density of the fermentation medium to at least about 100 g/L.

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