Immobilized titanium ion affinity chromatography material and its preparation and application

摘要

The present invention relates to an immobilized titanium ion affinity chromatography material and its preparation and application. Titanium ion is immobilized on solid support by taking advantage of strong interaction between titanium ions and phosphate groups on the solid support. The immobilized titanium ion affinity chromatography material is applied to enrich phosphopeptides. Phosphopeptides are isolated because they retained on the affinity material due to the strong chelating interaction between the immobilized titanium ions and the phosphate groups on the phosphopeptides.