| 发明名称 |
THERMOSTABLE BETA-GLUCOSIDASE |
| 摘要 |
A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including:
(A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1 or 2;(B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5; or(C) a polypeptide including an amino acid sequence having at least 80% sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5. |
| 申请公布号 |
US2016168553(A1) |
申请公布日期 |
2016.06.16 |
| 申请号 |
US201514964216 |
申请日期 |
2015.12.09 |
| 申请人 |
HONDA MOTOR CO., LTD. |
发明人 |
OKUMA Jiro;SUDA Migiwa;YAMAGUCHI Asuka;HIROSE Yoshitsugu;KONDO Yasuhiro;SATO Masaru;SHIBATA Daisuke |
| 分类号 |
C12N9/42;C12P19/02;C12N9/24;C12P19/14;C12N9/38 |
主分类号 |
C12N9/42 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A thermostable β-glucosidase comprising a β-glucosidase catalytic domain, the β-glucosidase catalytic domain comprising:
(A) a polypeptide comprising an amino acid sequence represented by SEQ ID NO: 1 or 2; (B) a polypeptide comprising an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5; or (C) a polypeptide comprising an amino acid sequence having at least 80% sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5. |
| 地址 |
Tokyo JP |
