Method for observing stem cells, method for removal of cell region in state tending toward differentiation, and device for observing stem cells

摘要

A method for observing stem cells by an observation device 1 comprises, placing stem cells C in a petri dish 11, mounting the petri dish 11 on a waveguide 21 via water 13, emitting illumination light L1 into the waveguide 21 and emitting the illumination light L1 to the stem cells C in the petri dish 11 via the water 13, and detecting scattered light L2, the scattered light L2 being the illumination light L1 emitted to the stem cells C that is scattered by the stem cells C and has passed through the waveguide 21. Then, in the light image detected by means of the scattered light L2, a region that is markedly darker than other regions is identified as being in the state tending toward differentiation.

主权项

1. A method for observing stem cells, comprising:
irradiating stem cells placed on a transparent member with oblique illumination light; capturing a scattered light image of the stem cells by a camera having a two dimensional pixel structure, in which a plurality of pixels are two-dimensionally arranged; and identifying a stem cell that is in the state tending toward differentiation in the sterm cells based on the scattered light image, wherein the irradiating irradiates the stem cells with the oblique illumination light via the transparent member.