Rapid cell purification systems

摘要

Methods and systems for purifying cells and/or viruses are provided. The sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the cells and/or viruses in the well. The cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the cells and/or viruses may be retained in the well by embedding in the medium. The medium including the embedded cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface.

主权项

1. A method of purifying at least one of cells and viruses in a sample comprising:
1) adding a sample containing contaminants and at least one of cells and viruses to a well disposed in a porous medium; 2) applying an electrical potential across the medium to cause the contaminants to enter the medium through one or more walls of said well while retaining the at least one of the cells and viruses in said well; 3) reversing the electrical potential for a sufficient time to displace the at least one of the cells and viruses from the surface of the medium; and 4) removing the at least one of the cells and viruses from said well.