Algal lipid compositions and methods of preparing and utilizing the same

摘要

This invention relates to compositions comprising high lipid content algae and methods of making and utilizing the same. In particular, the invention relates to high lipid content algae biomass and algal lipid materials derived from the same, methods of making the same, as well as to biofuels (e.g., biodiesel) and dietary compositions (e.g., animal feeds) comprising or made from the same. Compositions and methods of the invention find use in a variety of applications including biofuel, dietary (e.g., human and animal nutrition), therapeutic as well as research applications.

主权项

1. A process of making an algal biomass comprising at least 67% total fat comprising culturing an algae in two or more types of culture medium sequentially, and wherein said culturing comprises:
culturing the algae in a culture medium comprising a carbon source, yeast extract, a magnesium source, and a calcium source; and subsequently, culturing the algae in a culture medium comprising a carbon source, a yeast extract, a nitrogen source, a phosphate source, a magnesium source, a calcium source, and 4 g/L or less of sodium chloride, wherein the nitrogen and phosphate are in a ratio of 50:1 to 4:1, and wherein the type of algae is selected from the group consisting of Thraustochytrium, Schizochytrium, and Aurantiochytrium.