METHOD FOR PRODUCING COMPOSITE GEL BY CROSS-LINKING HYALURONIC ACID AND HYDROXYLPROPYL METHYLCELLULOSE

摘要

A method for covalently cross-linking hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC) by a diepoxide crosslinking agent. The method includes the following steps: a) mixing HA and HPMC in water; b) adding an alkali as a catalyst and a diepoxide as a crosslinking agent; c) neutralizing with hydrochloric acid and dehydrating with ethanol and acetone; and d) drying in vacuum and redissolving in water to obtain an HA-HPMC composite gel.

主权项

1. A crosslinked gel composition of hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), the crosslinked gel composition being produced by using HA and HPMC as raw material and crosslinking with 1, 2, 7, 8-diepoxyoctane (DEO) or 1, 4-butanediol diglycidyl ether (BDDE), wherein the specific producing method for the crosslinked gel composition is:
proportionally mixing HA and HPMC solution; after stirring thr reaction, adding sodium hydroxide solution into the mixed HA and HPMC solution until mixture is alkali; when well mixed, adding DEO or BDDE into the mixture; after stirring for reaction, adding acid until the mixture is week acidic; solidifying and dewatering the mixture using acetone; washing with organic solvent and then vacuum drying to produce HA-HPMC powder; and repeatedly dissolving the powder to produce HA-HPMC gel composition.