DE NOVO SYNTHESIZED GENE LIBRARIES

摘要

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

主权项

1. A method of synthesizing nucleic acids, comprising:
a) providing predetermined sequences for at least 750 preselected nucleic acids; b) providing a structure comprising a surface; c) synthesizing at least 100,000 non-identical polynucleotides extending from the surface; and d) assembling the at least 750 preselected nucleic acids, wherein each of the at least 750 preselected nucleic acids is at least 0.5 kb in length, wherein the at least 750 preselected nucleic acids comprise sequences encoded by the at least 100,000 non-identical polynucleotides, and wherein the assembled at least 750 preselected nucleic acids encode sequences with an aggregate error rate of less than 1 in 1000 bases compared to the predetermined sequences without correcting errors.