PROCESSING BLOOD

摘要

Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

主权项

1. An apparatus for processing blood, said apparatus comprising:
an inlet interface for coupling with a patient to receive blood directly from the circulation of said patient; a leukapheresis module coupled to said inlet interface for collecting bulk mononuclear blood cells from said received blood; an enrichment module coupled to said leukapheresis module for enriching concurrently target cells separated from non-target cells in said bulk mononuclear blood cells; a target-cell modification module adapted to provide modification selected from the group consisting of cytotoxic T lymphocyte (CTL) activation; T regulatory cell (Treq) activation and genetically modified blood cells protected from human immunodeficiency virus (HIV) coupled to at least one of said leukapheresis module and said enrichment module, said modification module modifying said enriched target cells; an outlet interface coupled to at least one of said leukapheresis module and said enrichment module for coupling with said patient to return enriched target cells to the circulation of said patient, said apparatus and said patient forming a closed loop when coupled together; and a controller for automated control of operation of said inlet and outlet interfaces, said leukapheresis module, and said enrichment module.