| 摘要 |
<p>The present invention relates generally to mutagenesis of target genes th at takes advantage of the natural mutagenic capabilities of B cells, and enh ances those capabilities by bringing the process of diversification under co ntrol. The invention provides a method for rapidly and inducibly generating point mutations and other types of diversification in expressed genes, such as antibody genes. This method can be coupled with selection to identify B c ell clones that produce, for example, antibodies of high affinity or specifi city. The diversification process can be modulated, accelerated, halted, swi tched between methods of mutagenesis and the like. The modulation of diversi fication in accordance with the invention is both inducible and reversible. The invention provides a means of rapid and feasible development of a repert oire of variant immunoglobulins and other polypeptides.</p> |
