TRANSFORMATION METHOD OF SOYBEAN USING MERISTEMATIC TISSUE OF GERMINATING SEED

摘要

A transformation method of soy bean is provided to produce the transgenic plant regardless of bean species and germination period, simplify the transformation process and save the expense. A method for transforming a soybean comprises: a step of culturing an agrobacterium which an expression vector containing a foreign target gene and marker gene for transforming the soybean is introduced and preparing suspension; a step of inoculating the suspension into embryo axis cross section of the soybean germination seed and co-culturing; a step of inducing transformed shoot in the co-cultured embryo axis cross section and selecting; a step of extending the selected shoot; and a step of inducing and purifying a root from the extended shoot.