ENZYMATIC AMINATION

摘要

A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.

主权项

1. A method, comprising:
(a) contacting a hydrocarbon comprising a hydroxyl group with a first polypeptide having an oxygen-dependent and cofactor-dependent carbohydrate oxidase activity or a whole-cell biocatalyst comprising the first polypeptide in the presence of oxygen and carbohydrate oxidase cofactor; and (b) contacting a hydrocarbon produced in the contacting (a) with a second polypeptide having a transaminase activity or a whole-cell biocatalyst comprising the second polypeptide and a third polypeptide having a cofactor-dependent amino acid dehydrogenase activity or a whole-cell biocatalyst comprising the third polypeptide in the presence of an amino acid dehydrogenase cofactor and a substrate amino acid for an amino acid dehydrogenase, wherein the hydrocarbon comprising a hydroxyl group comprises a 5 or 6 membered ring carrying at least one substituent of —(CH2)x—OH, where x is 0 to 4, and the first polypeptide is selected from the group consisting of an M1 variant of galactose oxidase from Fusarium NRRL 2903, pyranose oxidase from Phanerochaete chrysosporium, hexose oxidase from Chondrus crispus and a homologue thereof.