MUTATED INTERNAL RIBOSOMAL ENTRY SITE (IRES) FOR CONTROLLED GENE EXPRESSION

摘要

The present invention relates to a nucleic acid molecule comprising one or multiple mutant IRES elements. Further, the present invention relates to methods of enhancing gene expression and to methods of differentially controlling expression of one or multiple gene(s) of interest. In addition, the present invention relates to a kit for studying interactions or any application requiring co-expression of multiple genes.

主权项

1. Nucleic acid molecule comprising a first internal ribosome entry site (IRES) sequence, wherein the IRES sequence is a mutant IRES sequence and comprises or consists of a nucleotide sequence selected from the group consisting of any one of the nucleotides sequences set forth in SEQ ID Nos. 2-26.