| 摘要 |
The invention relates to an ATP binding molecule comprising a polypeptide which undergoes a conformational change from a first conformation to a second conformation upon binding of ATP, said polypeptide comprising amino acid sequence corresponding to at least amino acids 5 to 497 of SEQ ID NO:1, said polypeptide having at least 21% sequence identity to SEQ ID NO:1, said polypeptide having a value of RMSD <4 Å relative to RpMat B in the ATP-bound conformation, wherein said polypeptide comprises a cysteine residue for attachment of at least one reporter moiety, and comprises at least one reporter moiety attached thereto, wherein said cysteine residue for attachment of at least one reporter moiety is positioned such that said reporter moiety undergoes a change in fluorescence upon changing from said first conformation to said second conformation. The invention also relates to use of such a sensor, and to methods for assay of ATP. |
