ISOTHERMAL AMPLIFICATION METHOD FOR DETECTION OF NUCLEIC ACID MUTATION

摘要

A method is provided for detection of a nucleic acid region in a biological sample, by use of an isothermal amplification reaction, such as loop-mediated isothermal amplification (LAMP). In particular, the provided method is suitable for detection of the presence or absence of pathogenic organisms, such as the pathogenic JPs clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans. In addition, oligonucleotide primers are provided for performing the method. A kit is also provided for performing such methods. Moreover, a method for treatment of periodontitis is provided, wherein pathogenic organisms are detected by said isothermal amplification reaction followed by an antibiotic treatment.