发明名称 |
PRODUCTION METHOD FOR CHARGED NON-PROTEIN AMINO ACID-CONTAINING PEPTIDE |
摘要 |
An object of the present invention is to provide a method of producing a peptide containing a charged non-proteinogenic amino acid in a cell-free translation system, and the like. The present invention provides a method of producing a peptide containing a charged non-proteinogenic amino acid. It includes a step of expressing a peptide in a cell-free translation system including (i) at least one tRNA to which a non-proteinogenic amino acid having a protecting-group-introduced charged group has been bound and (ii) a nucleic acid that encodes the peptide and contains at least one codon corresponding to an anticodon of the tRNA; and a step of removing the protecting group of the non-proteinogenic amino acid residue contained in the peptide. |
申请公布号 |
EP3031915(A1) |
申请公布日期 |
2016.06.15 |
申请号 |
EP20140834909 |
申请日期 |
2014.08.04 |
申请人 |
THE UNIVERSITY OF TOKYO;PEPTIDREAM INC. |
发明人 |
MURAKAMI, HIROSHI;KAWAKAMI, TAKASHI;REID, PATRICK;SASAKI, TORU |
分类号 |
C12N15/67;C07K7/06;C12P21/02;G01N33/53;G01N33/68 |
主分类号 |
C12N15/67 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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