Salivary protein glycosylation test for diagnosis and monitoring of diabetes

摘要

Disclosed herein are methods and tests for diagnosing and/or monitoring a metabolic condition such as diabetes in a subject, wherein the methods and tests measure salivary glycoproteins. Some of the methods are based on the oxidation of glycoproteins in a sample from the subject, such as saliva or urine, for example using sodium metaperiodate, and then detecting the aldehydes generated during oxidation using a chemical detection method. Also disclosed are kits and lateral flow devices for detecting glycoproteins in a saliva sample.

主权项

1. A method of determining a glycemic state in a subject, the method comprising: chemically oxidizing a saliva sample from the subject, thereby oxidizing one or more glycoproteins in the saliva sample to generate one or more oxidation products, wherein the one or more oxidation products comprise one or more aldehydes;
quantifying the one or more aldehydes in the oxidized saliva sample; and comparing a level of the one or more aldehydes to a normal control value, wherein an increase in the level of the one or more aldehydes indicates that the subject has an elevated glycemic state.