发明名称 |
Method for In Vitro Quantifying Allo-Antibodies, Auto-Antibodies and/or Therapeutic Antibodies |
摘要 |
An in vitro method for quantifying a target-specific test antibody in a test sample, comprising the steps of: a) performing an immunoassay using a target immobilized on a support which is brought into contact with the test sample, the immunoassay comprising a step of measuring the binding of the target-specific test antibody to the immobilized target by using a detectable non-antibody ligand that binds to the Fc region or to a light chain of an antibody, whereby a concentration-related value of the target-specific test antibody in the test sample is obtained, and b) comparing the concentration-related value obtained at step a) with a reference value obtained by performing an immunoassay using the target immobilized on a support which is brought into contact with a calibration sample comprising a known concentration of a target-specific calibration antibody, the immunoassay comprising a step of measuring the binding of the target-specific calibration antibody to the immobilized target by using the detectable non-antibody ligand of step a), and wherein: (i) the target-specific test antibody of step a) and the target-specific calibration antibody of step b) are identical, or (ii) the target-specific test antibody of step a) and the target-specific calibration antibody of step b) are distinct. |
申请公布号 |
US2016216262(A1) |
申请公布日期 |
2016.07.28 |
申请号 |
US201414917791 |
申请日期 |
2014.09.11 |
申请人 |
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE ;UNIVERSITE PARIS DIDEROT (PARIS 7) ;UNIVERSITE PARIS DESCARTES ;UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6) ;ASSISTANCE PUBLIQUE HOPITAUX DE PARIS |
发明人 |
Dragon-Durey Marie-Agnes;Senant Marie |
分类号 |
G01N33/564;G01N33/94;G01N33/68 |
主分类号 |
G01N33/564 |
代理机构 |
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代理人 |
|
主权项 |
1. An in vitro method for quantifying a target-specific test antibody in a test sample, comprising the steps of:
a) performing an immunoassay using a target immobilized on a support which is brought into contact with the test sample, the immunoassay comprising a step of measuring the binding of the target-specific test antibody to the immobilized target by using a detectable non-antibody ligand that binds to the Fc region or a to light chain of an antibody, whereby a concentration-related value of the target-specific test antibody in the test sample is obtained, and b) comparing the concentration-related value obtained at step a) with a reference value obtained by performing an immunoassay using the target immobilized on a support which is brought into contact with a calibration sample comprising a known concentration of a target-specific calibration antibody, the immunoassay comprising a step of measuring the binding of the target-specific calibration antibody to the immobilized target by using the detectable non-antibody ligand of step a), and wherein: (i) the target-specific test antibody of step a) and the target-specific calibration antibody of step b) are identical, or (ii) the target-specific test antibody of step a) and the target-specific calibration antibody of step b) are distinct. |
地址 |
Paris FR |