Enzyme linked chemiluminescent assay

摘要

A sample containing an analyte is contacted with a ligand partner which is conjugated with an enzyme capable of catalysing a reaction or cascade of reactions to produce hydrogen peroxide, so that complexes of the analyte with the ligand partner/enzyme conjugate are formed. Uncomplexed ligand partner/enzyme conjugate may then be removed. The reaction or cascade of reactions is allowed to occur to produce an amount of hydrogen peroxide indicative of the ligand partner/enzyme present. A chemiluminescent substance is added under appropriate conditions to allow the presence or quantity of ligand partner/complex to be determined.