发明名称 |
METHOD OF APPLYING NON-GENETIC SUBSTANCE TO PERFORM SITE-DIRECTED REFORM OF PLANT GENOME |
摘要 |
A method of applying a non-genetic substance to perform site-directed reform of a plant genome. The method comprises the following steps: introducing a non-genetic substance into a cell or a tissue or a plant part of a target plant, the non-genetic substance being a nuclease specific to a target segment or an mRNA expressing the nuclease, the nuclease being a TALEN nuclease, a zinc finger nuclease (ZFN), clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) nuclease, or all nucleases capable of realizing genome editing. The nuclease acts to cut the target segment, and then site-directed reform of the target segment is completed via a DNA repair of the plant. |
申请公布号 |
WO2016155482(A1) |
申请公布日期 |
2016.10.06 |
申请号 |
WO2016CN76244 |
申请日期 |
2016.03.14 |
申请人 |
INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES |
发明人 |
GAO, Caixia;LIANG, Zhen;WANG, Yanpeng;SHAN, Qiwei;SONG, Qianna |
分类号 |
C12N15/01;A01H1/06;A01H5/00;C12N15/55;C12N15/87 |
主分类号 |
C12N15/01 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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