Nucleic-Acid-Sequence Determination Device and Nucleic-Acid-Sequence Determination Method

摘要

A nucleic-acid-sequence determination device equipped with two light sources having different wavelengths, two detectors, and an optical system for irradiating a sample with light from the two light sources and guiding fluorescent light from a nucleic acid in the sample to the two detectors. The optical system is provided with a dichroic mirror for causing the fluorescent light from the nucleic acid in the sample to split, and guiding the split light to the two detectors. The dichroic mirror has a transition wavelength from transmission to reflection in two locations, namely: between light-emitting bands of two types of short-wavelength fluorescent dye and light-emitting bands of two types of long-wavelength fluorescent dye.

主权项

1. A nucleic-acid-sequence determination device comprising:
two light sources having different wavelengths; two detectors; and an optical system for irradiating a sample with light from the two light sources and guiding fluorescent light from a nucleic acid in the sample to the two detectors, wherein the two light sources alternately illuminate the sample and modify the nucleic acid in the sample with two types of fluorescent dye that emit light in bandwidths between the wavelengths of the two light sources and two types of fluorescent dye that emit light in longer wavelength bands than those of the two light sources, and wherein the optical system is provided with a dichroic mirror for causing the fluorescent light from the nucleic acid in the sample to split, and guiding the split light to the two detectors, and the dichroic mirror has a transition wavelength from transmission to reflection in two locations, namely: between light-emitting bands of two types of short-wavelength fluorescent dye and between light-emitting bands of two types of long-wavelength fluorescent dye.