发明名称 |
METHOD FOR DETECTING, DIFFERENTIATING, AND QUANTIFYING T CELL POPULATIONS BY MEANS OF REVERSE TRANSCRIPTION QUANTITATIVE REAL-TIME PCR (RT-QPCR) TECHNOLOGY |
摘要 |
The present invention relates to a method for detection, differentiation and quantification of T cell populations, comprising the following steps a) contacting a first aliquot of a body fluid of an individual with at least one antigen, wherein the body fluid contains antigen presenting cells (APC) and T cells, b) incubating the first aliquot with at least one antigen for a certain period of time, c) detection and differentiation of the T cell population by detecting in the first aliquot and in a second aliquot of the body fluid of the individual, which has not been incubated with the at least one antigen, at least a first marker of the APC induced by T cells in a specific T cell population using reverse transcription quantitative real time-time polymerase chain reaction (RT-qPCR), and d) detection and quantification of the T cell population by determining the ratio of the detected marker of the APC of the first aliquot to the second aliquot as well as a kit for performing the method. |
申请公布号 |
EP2616816(B1) |
申请公布日期 |
2016.11.02 |
申请号 |
EP20110808564 |
申请日期 |
2011.09.16 |
申请人 |
LOPHIUS BIOSCIENCES GMBH |
发明人 |
DEML, LUDWIG;NAUMANN, KRISTINA;SCHLOMBS, KORNELIA;BARABAS, SASCHA |
分类号 |
G01N33/68;C12Q1/68;G01N33/50 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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