Concentration and purification of hydrophobins and antibodies with a phase separation method

摘要

The present invention relates to a purification and concentration method for proteins and antibodies. Particularly the present invention relates to a continuous surfactant based phase separation method for recovering hydrophobin fusion proteins, and for recovering target molecules, such as antibodies, directly from a liquid by using phase separation and hydrophobin-Protein A fusion technologies.

主权项

1. A method for purifying proteins with a continuous phase separation technology, wherein the method comprises the steps of:
a) mixing a surfactant and an aqueous protein solution, which contains at least one hydrophobin, a hydrophobin-like protein, or a fusion of any of these with a further molecule of interest, to form an extraction solution, b) allowing an aqueous phase and a surfactant phase to separate, and c) continuously removing a stream of the surfactant phase, containing at least one hydrophobin or a hydrophobin-like protein or a fusion of any of these with a further molecule of interest, and a stream of the aqueous phase from the extraction solution.