Apparatus for non-invasive in vivo measurement by raman spectroscopy

摘要

A metabolite concentration is measured in vivo using Raman spectroscopy in such a way as to receive at a detector (229) light scattered from the metabolite in interstitial fluid in skin in a measurement location (217) at a depth (218) of from 200-300 μm below the skin surface providing improved retention of correct calibration and transferability of calibration between individual subjects.

主权项

1. A method for non-invasive in vivo measurement by Raman spectroscopy of glucose present in interstitial fluid in the skin of a subject, comprising directing light from a light source into the skin via optical components defining a light path from said light source to a measurement location in the skin at a depth located at from 200 to 300 μm beneath the surface of the skin, receiving Raman scattered light back from the skin at a light detection unit via optical components defining a return path including using a skin engaging member having a distal surface for defining the position of said optical components defining the return path with respect to a surface of said skin and using said optical components defining a return path for Raman scattered light to transmit selectively to said light detection unit Raman scattered light scattered from near said measurement location of which at least 50% originates at depths from 60 to 400 μm beyond said distal surface of the skin engaging member, and deriving a measurement of glucose concentration at said measuring location from the Raman scattered light received at the light detection unit.