INTEGRATED DEVICE WITH EXTERNAL LIGHT SOURCE FOR PROBING DETECTING AND ANALYZING MOLECULES

摘要

System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.

主权项

1. An integrated device comprising:
a waveguide configured to propagate optical excitation energy; a sample well configured to receive a sample and positioned to receive the optical excitation energy from the waveguide; and a sensor positioned to receive light from the sample well and configured to generate a signal by aggregating, into at least two bins, charge carriers produced by received photons of luminescence emitted from the sample in response to the optical excitation energy, wherein the signal is indicative of lifetime of the luminescence.