发明名称 |
METHODS FOR THE REDUCTION OF STUTTER IN MICROSATELLITE AMPLIFICATION |
摘要 |
The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample wi th at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifyin g microsatellites having a G+C content of 50% or less, and methods of using al l of the foregoing. |
申请公布号 |
CA2446626(A1) |
申请公布日期 |
2002.11.14 |
申请号 |
CA20022446626 |
申请日期 |
2002.05.06 |
申请人 |
APPLERA CORPORATION |
发明人 |
BLOCH, WILL;COTICONE, SULEKHA RAO |
分类号 |
G01N33/53;C12N15/09;C12Q1/68;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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