发明名称 METHOD FOR PURIFICATION OF INTACT CELLS
摘要 The present invention relates to a novel method for cell selection, in which a chitin binding domain (ChBD) having high affinity to chitin which is an insoluble polymer of N-acetyl glucosamine or a cellulose binding domain (CBD) having high affinity to cellulose is imparted on surfaces of eukaryotic cells. More particularly, the present invention relates to a method for isolating transgenic eukaryotic cells, comprising the step of introducing an expression cassette including the ChBD or CBD to the eukaryotic cells and then selecting the introduced eukaryotic cells; a method for isolating plasma membranes; the expression cassette; a vector comprising the expression cassette; eukaryotic cells to which the vector is introduced; and a kit for isolating transgenic eukaryotic cells, comprising the expression cassette.
申请公布号 KR20130130563(A) 申请公布日期 2013.12.02
申请号 KR20120054473 申请日期 2012.05.22
申请人 SCRIPPS KOREA ANTIBODY INSTITUTE;THE SCRIPPS RESEARCH INSTITUTE 发明人 SONG BYEONG DOO;KIM, DAE HEE;LERNER RICHARD A.;PENG YINGJIE
分类号 C12N15/79;C12Q1/24 主分类号 C12N15/79
代理机构 代理人
主权项
地址