发明名称 |
KIND OF MUTATED PROTEINS A WITH HIGH ALKALI RESISTANCE FEATURE AND APPLICATION THEREOF |
摘要 |
A series of protein A mutants having high alkali resistance, and methods of using the protein A mutants are provided. The protein A mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions. The protein A mutants can be coupled to a solid support for immunoglobulin isolation, or conjugated to a label for immunoglobulin detection. This series of protein A mutants has high chemical stability under alkaline conditions of pH 13-14, and can also be used as chromatography ligands for purification procedures that use alkaline solutions under harsh conditions, such as Clean-In-Place (CIP). Also provided are methods of immunoglobulin separation and purification, and alkali regeneration of affinity chromatography medium that uses protein A as a ligand. |
申请公布号 |
US2016237124(A1) |
申请公布日期 |
2016.08.18 |
申请号 |
US201314778395 |
申请日期 |
2013.05.30 |
申请人 |
QIAN Hong;BAI Tao;HUA Rong;NANJING GENSCRIPT CO., LTD. |
发明人 |
QIAN Hong;BAI Tao;HUA Rong |
分类号 |
C07K14/31;C07K16/00;G01N33/68;C07K1/22 |
主分类号 |
C07K14/31 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Nanjing CN |