TAQ DNA POLYMERASES HAVING AN AMINO ACID SUBSTITUTION AT E681 AND HOMOLOGS THEREOF EXHIBITING IMPROVED SALT TOLERANCE

摘要

Thermostable DNA polymerases having an E410R substitution which result in a substantial improvement of signal uniformity compared to Taq DELTA 271/F272M/F667Y DNA polymerase. The instant DNA polymerases possess improved salt tolerance and have been shown to modulate the incorporation of terminators having a net positive or a net negative charge during the sequencing reaction.