Method for producing secreted proteins

摘要

The present invention discloses a method for producing a secreted biologically active mature α-galactosidase A that contains α2,6-sialic acid residues, comprising culturing an engineered mammalian cell that expresses α-galactoside α2,6-sialyltransferase such that the cell is capable of peptide sialylation, wherein the cell further comprises a nucleotide sequence that regulates gene expression operatively associated with an α-galactosidase A gene sequence under conditions in which the α-galactosidase A is sufficiently overexpressed by the engineered mammalian cell so as to be selectively secreted into the mammalian cell culture as a biologically active mature α-galactosidase A that contains α2,6-sialic acid residues; and isolating biologically active mature α-galactosidase A that contains α2,6-sialic acid residues from the mammalian cell culture.