METHOD OF TARGETING MISMATCHED DNA USING d8 SQUARE PLANAR METAL COMPLEXES

摘要

Disclosed are methods of using d8 square planar metal complexes containing tridentate π-conjugated ligands and ancillary ligand of N-heterocyclic carbene or di-phosphine ligand to target mismatched DNA. Targeting of mismatched DNA can be revealed by monitoring the differences in emission enhancement of metal complexes toward mismatched DNA and matched DNA; or the gradually heat release from isothermal titration calorimetry (ITC) when complexes bind toward mismatch DNA; or the significant increase in melting temperature of the mismatched DNA after adding complexes.

主权项

1. A method to target mismatched DNA, the method comprising
bringing into contact a DNA sample and a d8 square planar metal complex, and detecting the emission of the complex intercalated into DNA in the sample, wherein emission above a threshold level indicated the presence of mismatched DNA in the sample, wherein the complex has a structure according to Formula I or Formula II:wherein:
X̂ŶZ and X′̂Y′̂Z' are each, independently, a tridentate π-conjugated ligand; X, Y, Z, X′, Y′, and Z′ are each, independently, carbon or nitrogen; R, R′, and R″ are each, independently, a N-heterocyclic carbene ligand or a phosphine ligand; each M is a d8 metal atom; n is the charge of the complex; A is the counter-ion for the complex; b is the charge of the counter-ion; and y is the absolute value of n/b.