发明名称 |
SELECTIVE MODIFICATION OF POLYMER SUBUNITS TO IMPROVE NANOPORE-BASED ANALYSIS |
摘要 |
The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer. |
申请公布号 |
US2016222444(A1) |
申请公布日期 |
2016.08.04 |
申请号 |
US201414915611 |
申请日期 |
2014.09.02 |
申请人 |
UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION ;ILLUMINA, INC. |
发明人 |
Gundlach Jens H.;Laszlo Andrew;Derrington Ian;Mandell Jeffrey G. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method for analyzing a polymer analyte, comprising:
(a) translocating a polymer analyte comprising a modified subunit from a first conductive liquid medium to a second conductive liquid medium through a nanopore, wherein the nanopore provides liquid communication between the first conductive liquid medium and the second conductive liquid medium; (b) measuring an ion current between the first conductive liquid medium and the second conductive liquid medium as the polymer analyte passes through the nanopore; and (c) detecting the modified subunit based on the measured ion current. |
地址 |
Seattle WA US |