| 发明名称 |
SUBGROUP B RECOMBINANT HUMAN ADENOVIRUS VECTOR, AND METHODS FOR CONSTRUCTING AND FOR USING THE SAME |
| 摘要 |
A method for reconstructing a replication-selective oncolytic adenovirus using a subgroup B recombinant human adenovirus vector Ad11-5EP. The method includes: 1) deleting E1A CR2 gene and/or anti-apoptotic gene E1B 21K that is necessary for viability of an adenovirus in normal cells but not necessary in tumor cells; 2) inserting a tumor-specific promoter to drive the expression of E1A gene; 3) re-directing a cellular tropism of Ad11-5EP according to receptors on a tumor cell surface; or 4) allowing adenovirus to selectively replicate in tumor cells. |
| 申请公布号 |
US2016222413(A1) |
申请公布日期 |
2016.08.04 |
| 申请号 |
US201615098342 |
申请日期 |
2016.04.14 |
| 申请人 |
Beijing Bio-Targeting Therapeutics Technology Inc. |
发明人 |
WANG Yaohe;JIANG Guozhong;WONG Hanshi;CAO Fengyu;LEMOINE Nick |
| 分类号 |
C12N15/86;C12N7/00 |
主分类号 |
C12N15/86 |
| 代理机构 |
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代理人 |
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| 主权项 |
1. A method for reconstructing a replication-selective oncolytic adenovirus using a subgroup B recombinant human adenovirus vector Ad11-5EP (SEQ ID NO: 1), the method comprising one of the following steps:
1) deleting E1A CR2 (SEQ ID NO: 8) gene and/or anti-apoptotic gene E1B 21K (SEQ ID NO: 9) that is necessary for viability of an adenovirus in normal cells but not necessary in tumor cells; 2) inserting a tumor-specific promoter to drive the expression of E1A gene; 3) re-directing a cellular tropism of Ad11-5EP (SEQ ID NO: 1) according to receptors on a tumor cell surface; or 4) allowing adenovirus to selectively replicate in tumor cells. |
| 地址 |
Beijing CN |
