MODIFIED ZINC FINGER BINDING PROTEINS

摘要

Disclosed herein are compositions and method comprising non-canonical (e.g., non-C2H2) zinc finger proteins.

主权项

1. A plant comprising one or more plant cells in which a gamma tocopherol methyltransferase (gmt) gene is modified by transforming the one or more plant cells with a vector encoding a zinc finger nuclease comprising a pair of zinc finger proteins, each zinc finger protein comprising at least three zinc finger domains ordered finger 1 to finger 3 (F1 to F3), each zinc finger domain comprising a recognition helix region, wherein the zinc finger nuclease comprises a zinc finger protein comprising the following recognition helix regions:(i)F1: RSDNLAR;(SEQ ID NO: 40)F2: DRSNLTR;(SEQ ID NO: 41)andF3: RSDALTR;(SEQ ID NO: 42)or(ii)F1: RSDHLAR;(SEQ ID NO: 44)F2: TSGNLVR;(SEQ ID NO: 45)andF3: RSDHLRE;(SEQ ID NO: 46)or(iii)F1: RSDALTR;(SEQ ID NO: 48)F2: RSDHLTT;(SEQ ID NO: 49)andF3: RSDHLTT;(SEQ ID NO: 50)or(iv)F1: QSSNLAR;(SEQ ID NO: 52)F2: RSDNLAR;(SEQ ID NO: 53)andF3: QSGNLTR;(SEQ ID NO: 54)or(v)F1: RSDHLAR;(SEQ ID NO: 56)F2: QSGNLAR;(SEQ ID NO: 57)andF3: RSDNLTR;(SEQ ID NO: 58)or(vi)F1: ERGTLAR;(SEQ ID NO: 60)F2: QSGSLTR;(SEQ ID NO: 61)andF3: RSDHLTT;(SEQ ID NO: 62)or(vii)F1: RSDHLTQ;(SEQ ID NO: 64)F2: QSGNLAR;(SEQ ID NO: 65)andF3: RSDHLSR;(SEQ ID NO: 66)or(viii)F1: QSSHLAR;(SEQ ID NO: 68)F2: RSDNLAR;(SEQ ID NO: 69)andF3: QSGNLAR;(SEQ ID NO: 70)or(ix)F1: QSSNLQR;(SEQ ID NO: 72)F2: RSDNALR;(SEQ ID NO: 73)andF3: RSDNLQR;(SEQ ID NO: 74)or(x)F1: RSDNALR;(SEQ ID NO: 76)F2: RSDNLAR;(SEQ ID NO: 77)andF3: RSDNLTR;(SEQ ID NO: 78)or(xi)F1: QSSDLR;(SEQ ID NO: 80)F2: RSDELQR;(SEQ ID NO: 81)andF3: RSDALTR;(SEQ ID NO: 82)or(xii)F1: QSSNLAR;(SEQ ID NO: 84)F2: QSGHLQR;(SEQ ID NO: 85)andF3: RSDHLTT;(SEQ ID NO: 86)or(xiii)F1: QSSDLRR;(SEQ ID NO: 88)F2: QSGNLAR;(SEQ ID NO: 89)andF3: RSDNLTR;(SEQ ID NO: 90)or(xiv)F1: DRSHLTR;(SEQ ID NO: 92)F2: TSGHLTT;(SEQ ID NO: 93)andF3: QSSDLTR;(SEQ ID NO: 94)or(xvi)F1: QSSNLAR;(SEQ ID NO: 96)F2: RSDALSR;(SEQ ID NO: 97)andF3: QSGSLTR;(SEQ ID NO: 98)or(xvii)F1: QSSNLAR;(SEQ ID NO: 100)F2: RSDHLTT;(SEQ ID NO: 101)andF3: RSDALTR;(SEQ ID NO: 102)or such that activity of an expressed Gamma Tocopherol Methyltransferase (GMT) protein is modulated within the plant.