摘要 |
According to some aspects of the invention preparative methods and related compositions are provided for nucleic acid sequencing. In some embodiments, methods herein provide rapid amplification of template nucleic acids under isothermal conditions to produce samples that can be directly used with standard next generation nucleic acid sequencing systems, including, for example, high throughput flow cell based sequencing systems. In some embodiments, aspects of the invention relate to methods for preparing nucleic acids that involve exponentially amplifying nucleic acids under isothermal conditions for sequencing. |