Topographical Templating Of Polymeric Materials Using Cellular Morphology

摘要

Substrates for influencing the organization, spreading or adhesion of a selected cell to induce or stimulate growth, differentiation on regeneration of the cell or of tissue constituting the cells are provided as well as methods of making such substrates and methods of using such substrates.

主权项

1. A method of making an elastomeric polymer cell-templated substrate that supports growth or differentiation of a human neuron or glial cell or a tissue that includes the human neuron or glial cell, comprising the steps of:
a) placing a cell template in contact with a pre-polymer solution that is flowable in a liquid phase, wherein the cell template includes a selected human neuron or glial cell displayed on a surface of a solid support material; b) curing the pre-polymer solution to a rubbery or gelled solid to thereby form a polymerized film; and c) separating the polymerized film from the cell template, wherein the polymerized film, upon separation from the cell template, substantially reproduces microscopic dimensions and geometries of the selected human neuron or glial,whereby the elastomeric polymer cell-templated substrate, having a surface patterned with features having microscopic dimensions and geometries that substantially reproduce microscopic dimensions and geometries of the selected human neuron or glial cell, supports the growth or differentiation of the human neuron or glial cell or the tissue that includes the human neuron or glial cell.