IMPROVED E. COLI EXTRACT FOR PROTEIN SYNTHESIS

摘要

There is now a market for kits containing the necessary components for performing protein synthesis reactions using template DNA of the experimente r. One category of existing system for performing protein synthesis reactions i s based on an S-30 extract from the bacteria E. coli. It is disclosed here tha t a simple fractionation process can dramatically improve the performance of a n S-30 prokaryotic protein synthesis reaction mixture. In one embodiment, the fractionation is a simple freezing and thawing of an S-30 extract combined with a supplemental mix, followed by centrifugation. The resulting fractionated S-30 reaction mixture yields more full-length target protein an d lessnon-full length or non-target protein than possible using prior art S-30 systems.