NON-HUMAN MAMMALS FOR THE PRODUCTION OF CHIMERIC ANTIBODIES

摘要

The invention provides knock-in non-human cells and mammals having a genome encoding chimeric antibodies and methods of producing knock-in cells and mammals. Certain aspects of the invention include chimeric antibodies, humanized antibodies, pharmaceutical compositions and kits. Certain aspects of the invention also relate to diagnostic and treatment methods using the antibodies of the invention.

主权项

1. A mouse whose genome comprises an immunoglobulin (Ig) lambda light chain locus comprising a transgene comprising all or a portion of a human Igλ locus, the transgene comprising a DNA fragment comprising in operable linkage from 5′ to 3′ a DNA sequence of a human variable lambda (Vλ) segment gene operably linked to a constant region gene comprising a human Cλ and an Igλ 3′ locus control region (LCR), wherein the transgene comprising the human Igλ locus replaces all or a portion of the endogenous Igλ locus, thereby the mouse comprises a genome encoding the human Igλ light chain.