| 发明名称 |
SKIN EQUIVALENT CULTURE |
| 摘要 |
Disclosed is a method of preparing a collagenous construct comprising (i) casting viable collagen-producing human dermal fibroblasts in a support matrix comprising fibrin onto a support material, (ii) incubating in situ said support matrix with said viable collagen-producing human dermal fibroblasts in a collagen-inducing medium thereby (a) inducing or enhancing collagen production by said viable collagen-producing human dermal fibroblasts to form a collagenous construct, and (b) degrading said fibrin, (iii) rendering said collagenous construct free of said viable collagen-producing human dermal fibroblasts, and (iv) cross-linking said collagenous construct with a chemical or exposure to ultraviolet light. |
| 申请公布号 |
US2016333320(A1) |
申请公布日期 |
2016.11.17 |
| 申请号 |
US201615153499 |
申请日期 |
2016.05.12 |
| 申请人 |
SMITH & NEPHEW, INC. |
发明人 |
KEMP Paul;SHERING David;SHERING Andrew;JOHNSON Penny;MARSHALL Damian |
| 分类号 |
C12N5/071;A61L27/36;A61L27/24;A61L27/60;A61L27/20;A61L27/22;A61L27/38;A61L27/54 |
主分类号 |
C12N5/071 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of preparing a collagenous construct comprising:
(i) casting viable collagen-producing human dermal fibroblasts in a support matrix comprising fibrin onto a support material; (ii) incubating in situ said support matrix with said viable collagen-producing human dermal fibroblasts in a collagen-inducing medium thereby:
(a) inducing or enhancing collagen production by said viable collagen-producing human dermal fibroblasts to form a collagenous construct, and(b) degrading said fibrin; (iii) rendering said collagenous construct free of said viable collagen-producing human dermal fibroblasts; and (iv) cross-linking said collagenous construct with a chemical or exposure to ultraviolet light. |
| 地址 |
Memphis TN US |
